Here we demonstrate how the technology underlying Depixus MAGNA One™ can be used to determine the kinetic and thermodynamic parameters of an interaction at the single-molecule level. We use Depixus’ magnetic force spectroscopy to study the hybridization of short DNA oligonucleotides to single-stranded DNA and determine the kinetic and thermodynamic parameters. Furthermore, we show that…

Real-time single molecule data provides a deep understanding of protein-protein interactions (PPI) and can accelerate the development of drugs aimed at this emerging class of therapeutic targets. Our large-scale magnetic force spectroscopy (MFS) platform, Depixus MAGNA One™, delivers precise measurements of thousands of individual protein-protein interactions in parallel. These unique high-resolution data streams provide unprecedented…

From signal transduction to transcriptional regulation, protein-protein interactions (PPIs) form the basis of virtually every biological process and are major players in the dynamic game of life.   Over the past two decades, PPIs – particularly transient ones – have attracted growing attention as drug targets,1 thanks to their critical roles in biological regulation and disease.…

Based on magnetic force spectroscopy (MFS), Depixus MAGNA One™ is a user-friendly instrument system that allows real-time, label-free, non-destructive analysis of thousands of individual protein-protein interactions in parallel. In this case study we show how Depixus MAGNA One provides single molecule insights into interactions between an E3 ligase complex and its substrate. The detailed data…

In the world of molecular biology and drug development, understanding how biomolecules interact is fundamental.   Whether you’re validating a drug target, confirming a mechanism of action, or exploring structure–activity relationships, accurate analysis of biomolecular interactions is key to making informed decisions.  This is particularly true for emerging or complex modalities such as drugs targeting protein–protein…

Molecular glues are a promising new class of therapeutics for many diseases, but discovering and characterizing them is a challenge. By enabling researchers to measure thousands of individual protein interactions in real time, Depixus MAGNA One™ is set to revolutionize the development of these exciting novel drugs. Molecular glues – small molecules that facilitate protein-protein…

Most methods for analyzing biomolecular interactions produce averaged data from many thousands of molecules or more. A new generation of single-molecule biophysical techniques are transforming how we study biomolecular interactions, and can offer unparalleled insights into molecular heterogeneity, dynamic binding, and rare events. Understanding biomolecular interactions – the science of interactomics – is crucial for…

Magnetic Force Spectroscopy (MFS) is an analytical technique that directly probes the forces involved in individual biomolecular interactions. By taking this powerful approach and scaling it up to thousands of molecules in parallel, Depixus MAGNA One™ opens up a new world of insights into the intricate dynamics of biomolecular interactions that drive biological processes in…

Just as the advent of single-cell sequencing revolutionized genomics, Depixus MAGNA One™ is set to transform the field of interactomics. Built on magnetic force spectroscopy, this groundbreaking instrument enables unprecedented insights into thousands of individual biomolecular interactions. Breakthroughs in biology are driven by technological advances in scale and resolution Over the past two decades, we…

Protein-protein interactions (PPIs) lie at the heart of biology. Here we explain how Depixus MAGNA One™ can be used to analyze dynamic protein-protein interactions at scale, providing deep insights into biology and accelerating the development of novel therapeutics such as PPI inhibitors and molecular glues. What are protein-protein interactions and why are they important? Protein-protein…